ORIGINAL ARTICLE
Impact of some intravenously administered drugs (paracetamol, hydrocortisone, dexamethasone and amikacin) on coagulation hemostasis (in vitro evaluation study)
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College of Pharmacy, Basra University, Iraq
Online publication date: 2019-12-05
Publication date: 2019-12-05
Electron J Gen Med 2019;16(6):em167
KEYWORDS
TOPICS
ABSTRACT
Background:
Normally, the coagulation homeostasis prevents bleeding and retain the blood in the vascular system during periods of injury. Unfortunately, pathological factors, drugs or toxins could affect one or more stages of homeostasis mechanisms leading to bleeding or abnormal thrombi. Evaluation of prothrombin time is a favorable test for the screening of extrinsic coagulation mechanism whilst activated partial thromboplastin time measurement is a global coagulation screening method for intrinsic factor.
Material and Methods:
Blood samples were withdrawn from healthy adults volunteers for clotting times measurements from collected plasma. The effect of paracetamol, hydrocortisone, dexamethasone, and amikacin at different concentrations on coagulation homeostasis was in vitro investigated and compared to control and positive control.
Results:
High concentrations of all tested drugs except amikacin prolong significantly activated partial thromboplastin time, whilst the effect on the prothrombin time was conflicting.
Conclusions:
Paracetamol, hydrocortisone, dexamethasone, and amikacin could prolong clotting time expressed in prolongation of activated partial thromboplastin time comparable to the anticoagulants drugs.
REFERENCES (31)
2.
Choi JH, Kim DW, Park SE, Lee HJ, Kim KM, Kim KJ, et al. Anti-thrombotic effect of rutin isolated from Dendropanax morbifera Leveille. J Biosci Bioeng., 2015;120(2):181-6.
https://doi.org/10.1016/j.jbio... PMid:25777266.
4.
Schafer AI, Levine MN, Konkle BA, Kearon C. Thrombotic disorders: diagnosis and treatment. Hematology Am Soc Hematol Educ Program, 2003;520-39.
https://doi.org/10.1182/ashedu... PMid:14633797.
5.
Favaloro EJ, Lippi G. The new oral anticoagulants and the future of haemostasis laboratory testing. Biochem Medica, 2012;329–41. Available at:
http://www.biochemia-medica.co... PMid:23092064 PMCid:PMC3900050.
6.
Shetty S, Ghosh K, Mohanty D. Comparison of four commercially available activated partial thromboplastin time reagents using a semi-automated coagulometer. Blood Coagul Fibrinolysis, 2003 Jul;14(5):493-7.
https://doi.org/10.1097/000017... PMid:12851537.
8.
Girolami A, Cosi E, Tasinato V, Santarossa C, Ferrari S, Girolami B. Drug-Induced Thrombophilic or Prothrombotic States: An Underestimated Clinical Problem That Involves Both Legal and Illegal Compounds. Clin Appl Thromb, 2017 Oct 14;23(7):775-85. Available at:
http://journals.sagepub.com/do... PMid:27301402.
9.
Casonato A, Pontara E, Boscaro M, Sonino N, Sartorello F, Ferasin S, et al. Abnormalities of von Willebrand factor are also part of the prothrombotic state of Cushing’s syndrome. Blood Coagul Fibrinolysis, 1999 Apr;10(3):145-52.
https://doi.org/10.1097/000017... PMid:10357009.
11.
Park TK, Shin SJ, Lee JH. Effect of Drugs Associated With Bleeding Tendency on the Complications and Outcomes of Transforaminal Epidural Steroid Injection. Clin Spine Surg, 2017 Mar;30(2):E104-10.
https://doi.org/10.1097/BSD.00... PMid:27623303.
12.
Dootson GM, Keidan J, Harper PL. The influence of isotretinoin upon fibrinolysis in patients with acne. Br J Dermatol, 2006 Jul 29;133(1):66-70.
https://doi.org/10.1111/j.1365... PMid:7669643.
13.
Bettoli V, Pizzigoni S, Vasina S, Mancino A, Virgili A. Oral isotretinoin and haemophilia A. J Eur Acad Dermatology Venereol, 2004 Nov;18(6):718-20.
https://doi.org/10.1111/j.1468... PMid:15482307.
14.
Félix-Silva J, Souza T, Camara RBBG, Cabral B, Silva-Júnior AA, Rebecchi IM arin. M, et al. In vitro anticoagulant and antioxidant activities of Jatropha gossypiifolia L. (Euphorbiaceae) leaves aiming therapeutical applications. BMC Complement Altern Med, 2014 Dec 20;14(1):405.
https://doi.org/10.1186/1472-6... PMid:25328027 PMCid:PMC4210492.
15.
Halim A, Samama MM, Mendell J. Ex vivo reversal of the anticoagulant effects of edoxaban. Thromb Res, 2014 Oct;134(4):909-13.
https://doi.org/10.1016/j.thro... PMid:25179520.
17.
de Craen AJM, Di Giulio G, Lampe-Schoenmaeckers AJEM, Kessels AGH, Kleijnen J. Analgesic efficacy and safety of paracetamol-codeine combinations versus paracetamol alone: a systematic review. BMJ, 1996 Aug 10;313(7053):321-5.
https://doi.org/10.1136/bmj.31... PMid:8760737 PMCid:PMC2351742.
18.
Lopes RD, Horowitz JD, Garcia DA, Crowther MA, Hylek EM. Warfarin and acetaminophen interaction: a summary of the evidence and biologic plausibility. Blood, 2011 Dec 8;118(24):6269-73.
https://doi.org/10.1182/blood-... PMid:21911832 PMCid:PMC5524527.
19.
Dakheel S. Low Doses of Acetaminophen Prolonged Prothrombin Time and Increased International Normalized Ratio in Patients Receiving Long-Term Therapy. Clin Exp Pharmacol, 2015;05(05).
https://doi.org/10.4172/2161-1....
20.
Akinmoladun AC, Oguntunde KO, Owolabi LO, Ilesanmi OB, Ogundele JO, Olaleye MT, et al. Reversal of acetaminophen-generated oxidative stress and concomitant hepatotoxicity by a phytopharmaceutical product. Food Sci Hum Wellness, 2017 Mar;6(1):20-7.
https://doi.org/10.1016/j.fshw....
21.
Gray T, Hoffman RS, Bateman DN. Intravenous paracetamol – an international perspective of toxicity. Clin Toxicol, 2011 Mar 28;49(3):150-2.
https://doi.org/10.3109/155636... PMid:21443426.
22.
Brotman DJ, Girod JP, Posch A, Jani JT, Patel JV, Gupta M, et al. Effects of short-term glucocorticoids on hemostatic factors in healthy volunteers. Thromb Res, 2006 Jan;118(2):247-52.
https://doi.org/10.1016/j.thro... PMid:16005496.
23.
Barettino D, Masiá S, Montó F, Pérez P, Ocon PD, Moreno L, et al. Glucocorticoids as modulators of expression and activity of Antithrombin ( At ): Potential clinical relevance. Thromb Res, 2015;135(1):183-91.
https://doi.org/10.1016/j.thro... PMid:25466848.
24.
Calao A, Pivonello R, Faggiano A, et al. Persistence of increased cardiovascular risk in patients with cushing’s disease after five years of successful cure. J Clin Endocrinol Metab, 1999;84(8):2664-72.
https://doi.org/10.1210/jcem.8... PMid:10443657.
25.
Koutroumpi S, Spiezia L, Albiger N, et al. Thrombin generation in Cushing’s Syndrome: do the conventional clotting indices tell the whole truth? Pituitary, 2014 Feb 14;17(1):68-75.
https://doi.org/10.1007/s11102... PMid:23408210.
26.
von Känel R, Mausbach BT, Kudielka BM, Orth-Gomér K. Relation of morning serum cortisol to prothrombotic activity in women with stable coronary artery disease. J Thromb Thrombolysis, 2008;25(2):165-72.
https://doi.org/10.1007/s11239... PMid:17562129.
27.
Sneeboer MMS, Hutten BA, Majoor CJ, Bel EHD, Kamphuisen PW. Oral and inhaled corticosteroid use and risk of recurrent pulmonary embolism. Thromb Res, 2016;140:46-50.
https://doi.org/10.1016/j.thro... PMid:26897283.
28.
Isidori AM, Minnetti M, Sbardella E, Graziadio C, Grossman AB. Mechanisms in Endocrinology: The spectrum of haemostatic abnormalities in glucocorticoid excess and defect. Eur J Endocrinol, 2015 Sep;173(3):R101-13.
https://doi.org/10.1530/EJE-15... PMid:25987566.
29.
El-din MMG, Yassin NA. Blood Coagulation Changes in Response to Dexamethasone in the Normal Rat., 1994;62(3):625-30.
30.
Chen G, Fei X, Ling J. The Effects of Aminoglycoside Antibiotics on Platelet Aggregation and Blood Coagulation. Clin Appl Thromb, 2012 Sep 12;18(5):538-41.
https://doi.org/10.1177/107602... PMid:22327814.
31.
Dinev T, Hubenov H, Lashev L, Physiology V, Zagora S. Effects of Aminoglycoside and Aminocyclitol Antibiotics on Blood Coagulation in Goats, 2006;9(4):293-7.