Biological activities and chemical composition of essential oil isolated from Artemisia herba-alba
More details
Hide details
Department of Medical Analysis, Princess Aisha Bint Al-Hussein College of Nursing and Medical Sciences, Al-Hussein Bin Talal University, Ma’an 71111, JORDAN
Department of Medical Laboratory Sciences, Mutah University, Mutah, JORDAN
Department of Chemistry, Science College, Al-Hussein Bin Talal University, Ma’an, JORDAN
Department of Pharmacology, International Medical School, Management and Science University, Shah Alam, Selangor, MALAYSIA
Department of Pharmacy, Faculty of Medicine and Health Sciences, University of Science and Technology, Aden, YEMEN
Publication date: 2024-01-26
Electron J Gen Med 2024;21(1):em569
Artemisia herba-alba (AHA), known as sheeh in Jordan, is recommended by regional traditional healers for the treatment of a variety of diseases. AHA has been used in folk medicine to treat colds, coughing, bronchitis, intestinal disturbances, diarrhea, neuralgias, arterial hypertension, and diabetes. The objectives of the current study were to identify the chemical compositions of the essential oil extracted from dried leaf powder of AHA cultivated in Jordan and investigate its antibacterial and antioxidant activities. The essential oil was isolated using hydro distillation, and the identification of artemisia herba-alba essential oil (AHEO) composition was performed using validated gas chromatography-mass spectrometry (GC-MS). The antibacterial activity of AHEO was assessed against escherichia coli, pseudomonas aeruginosa, klebsiella pneumonia, and staphylococcus aureus and two clinical isolates (methicillin-resistant staphylococcus aureus and methicillin-resistant staphylococcus epidermidis [MRSE]) using a disc diffusion method, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) values, using the micro-dilution broth method. Additionally, antioxidant activities were determined using DPPH and ABTS radical scavenging assays. The results revealed that the yield of AHEO was 4.41% v/w, with nearly 22 identified compounds, constituting approximately 96.80% of the total mass of essential oils. Monoterpenoids was the major compounds (71.90%), with alpha pinene being the major component, accounting for 17.20% of the composition. The total phenolic and flavonoid contents were 43.97 mg GAE/g and 30.11 mg CE/g, respectively. The antibacterial activity of AHEO against MRSE exhibited the highest inhibitory effect, while E.coli showed the highest MBC value. Furthermore, AHEO demonstrated significant antioxidant activity (IC50= 64.57 and 34.01 for DPPH and ABTS, respectively). The results indicate that AHEO possess good antioxidant and antibacterial properties, suggesting that they may be used as a supplementary food and antimicrobial agent.
Maugeri G, Lychko I, Sobral R, Roque ACA. Identification and antibiotic-susceptibility profiling of infectious bacterial agents: A review of current and future trends. Biotechnol J. 2019;14(1):e1700750. https://doi.org/10.1002/biot.2... PMid:30024110 PMCid:PMC6330097.
Dmour SM, Qaralleh H, Al-Limoun M, et al. Combined antibacterial activity of eucalyptol, γ-terpinene, p-cymol and punicalagin with cefotaxime against methicillin (oxacillin) resistant staphylococcus aureus isolate. Res J Pharm Technol. 2022;15(9):3905-11. https://doi.org/10.52711/0974-....
Elsonbaty SM, Eltahawy N, Al-Dmour SM, Easa S, Qaralleh H. Ionizing radiation effect on teucrium polium: Phytochemical contents antioxidant and antibacterial activity. Arab J Nucl Sci Appl. 2020;53(2):98-110. https://doi.org/10.21608/ajnsa....
Hussein RA, El-Anssary AA. Plants secondary metabolites: The key drivers of the pharmacological actions of medicinal plants. In: Builders PF, editor. Herbal medicine. IntechOpen; 2018. https://doi.org/10.5772/intech....
Abduh MS, Saghir SAM, Al Hroob AM, et al. Averrhoa carambola leaves prevent dyslipidemia and oxidative stress in a rat model of poloxamer-407-induced acute hyperlipidemia. Front Pharmacol. 2023;14:1134812. https://doi.org/10.3389/fphar.... PMid:36814487 PMCid:PMC9939629.
Ivanišová E, Kačániová M, Savitskaya TA, Grinshpan DD. Medicinal herbs: Important source of bioactive compounds for food industry. In: Ahmad RS, editor. Herbs and spices–New processing technologies. IntechOpen; 2021. https://doi.org/10.5772/intech....
Kar S, Gupta P, Gupta J. Essential oils: Biological activity beyond aromatherapy. Nat Prod Sci. 2018;24(3):139-47. https://doi.org/10.20307/nps.2....
Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils–A review. Food Chem Toxicol. 2008; 46(2):446-75. https://doi.org/10.1016/j.fct.... PMid:17996351.
Radulovic N, Blagojevic P, Stojanovic-Radic Z, Stojanovic N. Antimicrobial plant metabolites: Structural diversity and mechanism of action. Curr Med Chem. 2013;20(7):932-52. https://doi.org/10.2174/092986... PMid:23210781.
de Sousa DP, Damasceno ROS, Amorati R, et al. Essential oils: Chemistry and pharmacological activities. Biomolecules. 2023;13(7):1144. https://doi.org/10.3390/biom13... PMid:37509180 PMCid:PMC10377445.
Ouhayoun JP. Penetrating the plaque biofilm: Impact of essential oil mouthwash. J Clin Periodontol. 2003;30(Suppl 5):10-2. https://doi.org/10.1034/j.1600... PMid:12787196.
Buhagiar JA, Podesta MT, Wilson AP, Micallef MJ, Ali S. The induction of apoptosis in human melanoma, breast and ovarian cancer cell lines using an essential oil extract from the conifer tetraclinis articulata. Anticancer Res. 1999;19(6B):5435-43.
Chen L, Deng H, Cui H, et al. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget. 2018;9(6):7204-18. https://doi.org/10.18632/oncot... PMid:29467962 PMCid:PMC5805548.
Sinico C, De Logu A, Lai F, et al. Liposomal incorporation of artemisia arborescens L. essential oil and in vitro antiviral activity. Eur J Pharm Biopharm. 2005;59(1):161-8. https://doi.org/10.1016/j.ejpb... PMid:15567314.
Koo B-S, Lee S-I, Ha J-H, Lee D-U. Inhibitory effects of the essential oil from SuHeXiang Wan on the central nervous system after inhalation. Biol Pharm Bull. 2004;27(4):515-9. https://doi.org/10.1248/bpb.27... PMid:15056857.
Bordia A. Effect of garlic on blood lipids in patients with coronary heart disease. Am J Clin Nutr. 1981;34(10):2100-3. https://doi.org/10.1093/ajcn/3... PMid:7293938.
Hamid A, Aiyelaagbe O, Usman L. Essential oils: Its medicinal and pharmacological uses. Int J Curr Res. 2011;33(2):86-98.
Panizzi L, Flamini G, Cioni P, Morelli I. Composition and antimicrobial properties of essential oils of four Mediterranean lamiaceae. J Ethnopharmacol. 1993;39(3): 167-70. https://doi.org/10.1016/0378-8... PMid:8258973.
Smith-Palmer A, Stewart J, Fyfe L. Antimicrobial properties of plant essential oils and essences against five important food-borne pathogens. Lett Applied Microbiol. 1998;26(2): 118-22. https://doi.org/10.1046/j.1472... PMid:9569693.
Filgueiras CT, Vanetti MCD. Effect of eugenol on growth and listeriolysin O production by listeria monocytogenes. Braz Arch Biol Technol. 2006;49:405-9. https://doi.org/10.1590/S1516-....
Daifas DP, Smith JP, Blanchfield B, Sanders G, Austin JW, Koukoutisis J. Effects of mastic resin and its essential oil on the growth of proteolytic clostridium botulinum. Int J Food Microbiol. 2004;94(3):313-22. https://doi.org/10.1016/j.ijfo... PMid:15246242.
de Souza EL, de Barros JC, de Oliveira CEV, da Conceição ML. Influence of origanum vulgare L. essential oil on enterotoxin production, membrane permeability and surface characteristics of staphylococcus aureus. Int J Food Microbiol. 2010;137(2-3):308-11. https://doi.org/10.1016/j.ijfo... PMid:20015563.
Rammanee K, Hongpattarakere T. Effects of tropical citrus essential oils on growth, aflatoxin production, and ultrastructure alterations of aspergillus flavus and aspergillus parasiticus. Food Bioprocess Technol. 2011;4:1050-9. https://doi.org/10.1007/s11947....
Swamy MK, Akhtar MS, Sinniah UR. Antimicrobial properties of plant essential oils against human pathogens and their mode of action: An updated review. J Evid Based Complementary Altern Med. 2016;2016:3012462. https://doi.org/10.1155/2016/3... PMid:28090211 PMCid:PMC5206475.
Diass K, Oualdi I, Dalli M, et al. Artemisia herba alba essential oil: GC/MS analysis, antioxidant activities with molecular docking on S protein of SARS-CoV-2. Indones J Sci Technol. 2023;8(1):1-18. https://doi.org/10.17509/ijost....
Mohamed AE-HH, El-Sayed M, Hegazy ME, Helaly SE, Esmail AM, Mohamed NS. Chemical constituents and biological activities of artemisia herba-alba. Rec Nat Prod. 2010;4(1):1-25.
Younsi F, Trimech R, Boulila A, et al. Essential oil and phenolic compounds of artemisia herba-alba (asso.): Composition, antioxidant, antiacetylcholinesterase, and antibacterial activities. Int J Food Prop. 2016;19(7):1425-38. https://doi.org/10.1080/109429....
Houti H, Ghanmi M, Satrani B, et al. Moroccan endemic artemisia herba-alba essential oil: GC-MS analysis and antibacterial and antifungal investigation. Separations. 2023;10(1):59. https://doi.org/10.3390/separa....
Mohamed HR, Amer M, Faky ASAE. Growth retardation and apoptotic death of tumor cells by artemisia herba-alba oral administration in ehrlich solid carcinoma bearing mice. Braz J Pharmacogn. 2020;29(6):763-72. https://doi.org/10.1016/j.bjp.....
Abu-Darwish MS, Cabral C, Gonçalves MJ, et al. Artemisia herba-alba essential oil from Buseirah (South Jordan): Chemical characterization and assessment of safe antifungal and anti-inflammatory doses. J Ethnopharmacol. 2015;174:153-60. https://doi.org/10.1016/j.jep.... PMid:26277492.
Réggami Y, Benkhaled A, Boudjelal A, et al. Artemisia herba-alba aqueous extract improves insulin sensitivity and hepatic steatosis in rodent model of fructose-induced metabolic syndrome. Arch Physiol Biochem. 2021;127(6): 541-50. https://doi.org/10.1080/138134... PMid:31464524.
Sadeghimahalli F, Khaleghzadeh-Ahangar H, Baluchnejadmojarad T. Role of prostaglandins in the vasodilator effect of the aqueous extract from artemisia annua plant in streptozotocin-induced diabetic rats. Annu Res Rev Biol. 2019;31(6):1-10. https://doi.org/10.9734/arrb/2....
Shaapan RM, Al-Abodi HR, Alanazi AD, et al. Myrtus communis essential oil; anti-parasitic effects and induction of the innate immune system in mice with toxoplasma gondii infection. Molecules. 2021;26(4):819. https://doi.org/10.3390/molecu... PMid:33557392 PMCid:PMC7915315.
AM Saghir S, Sadikun A, Al-Suede FS, MSA Majid A, Murugaiyah V. Antihyperlipidemic, antioxidant and cytotoxic activities of methanolic and aqueous extracts of different parts of star fruit. Curr Pharm Biotechnol. 2016;17(10):915-25. https://doi.org/10.2174/138920... PMid:27262321.
Alsohaili SA, Al-fawwaz AT. Composition and antimicrobial activity of achillea fragrantissima essential oil using food model media. Eur Sci J. 2014;10(30):156-65.
Adams RP. Identification of essential oil components by gas chromatography/mass spectrometry. Gruver, TX, USA: Texensis Publishing; 2017.
Qaralleh H, Idid S, Saad S, Susanti D, Taher M, Khleifat K. Antifungal and antibacterial activities of four Malaysian sponge species (petrosiidae). J Mycol Med. 2010;20(4):315-20. https://doi.org/10.1016/j.mycm....
Bauer A, Kirby W, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966;45(4_ts):493-6. https://doi.org/10.1093/ajcp/4... PMid:5325707.
McDonald A, Mascagni F. Colocalization of calcium-binding proteins and GABA in neurons of the rat basolateral amygdala. Neuroscience. 2001;105(3):681-93. https://doi.org/10.1016/S0306-... PMid:11516833.
Polshettiwar S, Ganjiwale R, Wadher S, Yeole P. Spectrophotometric estimation of total tannins in some ayurvedic eye drops. Indian J Pharm Sci. 2007;69(4):574-6. https://doi.org/10.4103/0250-4....
Bajpai VK, Baek K-H, Kang SC. Control of salmonella in foods by using essential oils: A review. Food Res Int. 2012;45(2):722-34. https://doi.org/10.1016/j.food....
Cakir A, Kordali S, Zengin H, Izumi S, Hirata T. Composition and antifungal activity of essential oils isolated from hypericum hyssopifolium and hypericum heterophyllum. Flavour Fragr J. 2004;19(1):62-8. https://doi.org/10.1002/ffj.12....
Al-Shuneigat J, Al-Sarayreh S, Al-Qudah M, Al-Tarawneh I, Al-Saraireh Y, Al-Qtaitat A. GC-MS analysis and antibacterial activity of the essential oil isolated from wild artemisia herba-alba grown in South Jordan. Br J Med Med Res. 2015;5(3):297. https://doi.org/10.9734/BJMMR/....
Amin SM, Hassan HM, El Gendy AENG, et al. Comparative chemical study and antimicrobial activity of essential oils of three artemisia species from Egypt and Saudi Arabia. Flavour Fragr J. 2019;34(6):450-9. https://doi.org/10.1002/ffj.35....
Amor G, Caputo L, La Storia A, De Feo V, Mauriello G, Fechtali T. Chemical composition and antimicrobial activity of artemisia herba-alba and origanum majorana essential oils from Morocco. Molecules. 2019;24(22):4021. https://doi.org/10.3390/molecu... PMid:31698834 PMCid:PMC6891654.
Mohsen H, Ali F. Essential oil composition of artemisia herba-alba from southern Tunisia. Molecules. 2009; 14(4):1585-94. https://doi.org/10.3390/molecu... PMid:19384287 PMCid:PMC6254365.
Chen X, Shang S, Yan F, et al. Antioxidant activities of essential oils and their major components in scavenging free radicals, inhibiting lipid oxidation and reducing cellular oxidative stress. Molecules. 2023;28(11):4559. https://doi.org/10.3390/molecu... PMid:37299039 PMCid:PMC10254587.
Miguel MG. Antioxidant and anti-inflammatory activities of essential oils: A short review. Molecules. 2010;15(12):9252-87. https://doi.org/10.3390/molecu... PMid:21160452 PMCid:PMC6259136.
Amorati R, Foti MC, Valgimigli L. Antioxidant activity of essential oils. J Agric Food Chem. 2013;61(46):10835-47. https://doi.org/10.1021/jf4034... PMid:24156356.
Cheli F, Baldi A. Nutrition-based health: Cell-based bioassays for food antioxidant activity evaluation. J Food Sci. 2011;76(9):R197-205. https://doi.org/10.1111/j.1750... PMid:22416720.
Nazzaro F, Fratianni F, De Martino L, Coppola R, De Feo V. Effect of essential oils on pathogenic bacteria. Pharmaceuticals. 2013;6(12):1451-74. https://doi.org/10.3390/ph6121... PMid:24287491 PMCid:PMC3873673.
Holley RA, Patel D. Improvement in shelf-life and safety of perishable foods by plant essential oils and smoke antimicrobials. Food Microbiol. 2005;22(4):273-92. https://doi.org/10.1016/j.fm.2....
Raut JS, Karuppayil SM. A status review on the medicinal properties of essential oils. Ind Crops Prod. 2014;62:250-64. https://doi.org/10.1016/j.indc....
Hammer KA, Carson CF. Antibacterial and antifungal activities of essential oils. In: Thormar H, editor. Lipids and essential oils as antimicrobial agents. Wiley; 2011. p. 255-306. https://doi.org/10.1002/978047....
Oussalah M, Caillet S, Lacroix M. Mechanism of action of Spanish oregano, Chinese cinnamon, and savory essential oils against cell membranes and walls of escherichia coli O157: H7 and listeria monocytogenes. J Food Prot. 2006;69(5):1046-55. https://doi.org/10.4315/0362-0... PMid:16715803.
Saad NY, Muller CD, Lobstein A. Major bioactivities and mechanism of action of essential oils and their components. Flavour Fragr J. 2013;28(5):269-79. https://doi.org/10.1002/ffj.31....
Turina AdV, Nolan M, Zygadlo J, Perillo M. Natural terpenes: Self-assembly and membrane partitioning. Biophys Chem. 2006;122(2):101-13. https://doi.org/10.1016/j.bpc.... PMid:16563603.
Cox SD, Gustafson JE, Mann CM, et al. Tea tree oil causes K+ leakage and inhibits respiration in escherichia coli. Lett Appl Microbiol. 1998;26(5):355-8. https://doi.org/10.1046/j.1472... PMid:9674165.
Cox SD, Mann CM, Markham JL, et al. The mode of antimicrobial action of the essential oil of melaleuca alternifolia (tea tree oil). J Appl Microbiol. 2000;88(1):170-5. https://doi.org/10.1046/j.1365... PMid:10735256.
Longbottom CJ, Carson CF, Hammer KA, Mee BJ, Riley TV. Tolerance of pseudomonas aeruginosa to melaleuca alternifolia (tea tree) oil is associated with the outer membrane and energy-dependent cellular processes. J Antimicrob Chemother. 2004;54(2):386-92. https://doi.org/10.1093/jac/dk... PMid:15254026.
Burt S. Essential oils: Their antibacterial properties and potential applications in foods–A review. Int J Food Microbiol. 2004;94(3):223-53. https://doi.org/10.1016/j.ijfo... PMid:15246235.
Salido S, Valenzuela LR, Altarejos J, Nogueras M, Sánchez A, Cano E. Composition and infraspecific variability of artemisia herba-alba from Southern Spain. Biochem Syst Ecol. 2004;32(3):265-77. https://doi.org/10.1016/j.bse.....
Feuerstein I, Danin A, Segal R. Constitution of the essential oil from an artemisia herba-alba population of Spain. Phytochemistry. 1988;27(2):433-4. https://doi.org/10.1016/0031-9....
Rekkab S, Abaza I, Chibani S, Kabouche A, Kabouche Z. Chemical composition of the essential oil of aerial parts of artemisia herba-alba asso. from Oum El-Bouaghi (Algeria) and chemotaxonomic survey. J Mater Environ Sci. 2016;7(12):4383-90.
da Silva Rivas AC, Lopes PM, de Azevedo Barros MM, Costa Machado DC, Alviano CS, Alviano DS. Biological activities of α-pinene and β-pinene enantiomers. Molecules. 2012; 17(6):6305-16. https://doi.org/10.3390/molecu... PMid:22634841 PMCid:PMC6268778.
Lakehal S, Meliani A, Benmimoune S, Bensouna S, Benrebiha F, Chaouia C. Essential oil composition and antimicrobial activity of artemisia herba-alba asso grown in Algeria. Med Chem. 2016;6(6):435-9. https://doi.org/10.4172/2161-0....
Cheraif K, Bakchiche B, Gherib A, et al. Chemical composition, antioxidant, anti-tyrosinase, anti-cholinesterase and cytotoxic activities of essential oils of six Algerian plants. Molecules. 2020;25(7):1710. https://doi.org/10.3390/molecu... PMid:32276465 PMCid:PMC7181011.
Journals System - logo
Scroll to top