Aim: The aim of the study is to evaluate the oxidant and antioxidant balance in experimentally induced testicular injury by ischemia reperfusion in rats with different time intervals of torsion/detorsion of the spermatic cord. Method: Male rats were divided into thirteen groups and each group containing six rats. All rats were subjected to right spermatic cord torsion followed by detorsion for different time intervals except the sham control group. Result: Malondialdehyde, super oxide dismutase, catalase levels were estimated in testicular tissue of each rat. Spermatic cord torsion/ detorsion induced a significant increase in testicular malondialdehyde contents and significant decrease in SOD, catalase levels when compared to sham control group in a time dependent manner. Conclusion: Our findings clearly represents that torsion for 4 hours followed by detorsion for 4 hours induces the highest oxidative stress with a great extent of lipid peroxidation and drastic reduction of SOD and catalase levels of the testis and is, therefore, a valid model for studying the oxidative antioxidant effects of the ischemia/ reperfusion injury in rat testis.