Current issue
Archive
About the Journal
About us
Aims and Scope
Indexing and Abstracting
Editorial Office
Open Access Policy
Publication Ethics
Contact
For Authors
Editorial Policy
Peer Review Policy
Manuscript Preparation Guidelines
Copyright & Licensing
Publication Fees
Fast-Track Paper Publication Option
Conflict Interest Guidance
Submit an Article
Special Issues
News & Editorials
“Fake,” “Predatory,” and “Pseudo” Journals: Charlatans Threatening Trust in Science
Shifting the Journal submission-review system to the Editorial System Manuscript December 27, 2017.
ORIGINAL ARTICLE
Evaluation of cytomegalovirus DNA in periymphatic fluid in patients with sensorineural hearing loss using PCR technique
| 1 | Associate Professor of Otolaryngology. Hearing Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran |
| 2 | Professor of Virology. Health Research Institute, Infectious and Tropical Disease Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran |
| 3 | Specialist Otolaryngology. Delhi, India |
| 4 | PhD of Virology. Health Research Institute, Infectious and Tropical Disease Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran |
| 5 | Resident of Otolaryngology. Hearing Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran |
Online publication date: 2018-08-12
Publication date: 2019-04-17
Electron J Gen Med 2019;16(2):em113
KEYWORDS
ABSTRACT
Purpose:
Congenital cytomegalovirus (CMV) infection is the most common congenital infection in developing countries with sensorineural hearing loss (SNHL) being the most important of its long-term sequelae. The aim of this research was to evaluate the amount of CMV DNA in perilymph of patients with SNHL undergoing cochlear implant (CI) procedure by Polymerase chain reaction (PCR) technique.
Materials and Methods:
This cross-sectional and prospective study was conducted in a group of patients with ages ranging from 1 to 7 years. The perilymph was extracted preoperatively using a Yale spinal needle after opening of the round window. 25 microliters of perilymph was extracted and stored at -80 degrees centigrade. PCR technique was employed to detect CMV DNA. The data was collected and statistically analyzed.
Results:
A sample size of 41 patients with bilateral profound SNHL was selected. After cochlear implantation, CMV DNA evaluation was performed which was found positive in 18 patients (43.9%) and negative in 23 patients (56.09%) with a statistically significant relationship. No statistical relationship was found with gender, age and other comorbid disorders. 3-6 months’ follow-up was individually performed to compare Categories of Auditory Performance (CAP) and Speech Intelligibility Rating (SIR) tests in CMV DNA positive and negative patients, yielding different results which were statistically insignificant.
Conclusion:
The early detection of CMV infection with real time PCR technique enables us to perform viral screening tests in patients with SNHL.
Congenital cytomegalovirus (CMV) infection is the most common congenital infection in developing countries with sensorineural hearing loss (SNHL) being the most important of its long-term sequelae. The aim of this research was to evaluate the amount of CMV DNA in perilymph of patients with SNHL undergoing cochlear implant (CI) procedure by Polymerase chain reaction (PCR) technique.
Materials and Methods:
This cross-sectional and prospective study was conducted in a group of patients with ages ranging from 1 to 7 years. The perilymph was extracted preoperatively using a Yale spinal needle after opening of the round window. 25 microliters of perilymph was extracted and stored at -80 degrees centigrade. PCR technique was employed to detect CMV DNA. The data was collected and statistically analyzed.
Results:
A sample size of 41 patients with bilateral profound SNHL was selected. After cochlear implantation, CMV DNA evaluation was performed which was found positive in 18 patients (43.9%) and negative in 23 patients (56.09%) with a statistically significant relationship. No statistical relationship was found with gender, age and other comorbid disorders. 3-6 months’ follow-up was individually performed to compare Categories of Auditory Performance (CAP) and Speech Intelligibility Rating (SIR) tests in CMV DNA positive and negative patients, yielding different results which were statistically insignificant.
Conclusion:
The early detection of CMV infection with real time PCR technique enables us to perform viral screening tests in patients with SNHL.
REFERENCES (17)
1.
Saki N, Bayat A, Hoeinabadi R, Nikakhlagh S, Karimi M, Dashti R. Universal newborn hearing screening in southwestern Iran. International Journal of Pediatric Otorhinolaryngology; 2017;97:89-92. https://doi.org/10.1016/j.ijpo... PMid:28483258.
2.
Atkinson C, Walter S, Sharland M, Tookey P, Luck S, Peckham C, Griffiths P. Use of stored dried blood spots for retrospective diagnosis of congenital CMV. Journal of medical virology. 2009 Aug 1;81(8):1394-8. https://doi.org/10.1002/jmv.21... PMid:19551829.
3.
Cannon MJ, Hyde TB, Schmid DS. Review of cytomegalovirus shedding in bodily fluids and relevance to congenital cytomegalovirus infection. Reviews in medical virology. 2011 Jul 1;21(4):240-55. https://doi.org/10.1002/rmv.69... PMid:21674676 PMCid:PMC4494736.
4.
Park AH, Duval M, McVicar S, Bale JF, Hohler N, Carey JC. A diagnostic paradigm including cytomegalovirus testing for idiopathic pediatric sensorineural hearing loss. The Laryngoscope. 2014 Nov 1;124(11):2624-9. https://doi.org/10.1002/lary.2... PMid:24965608.
5.
Sugiura S, Yoshikawa T, Nishiyama Y, Morishita Y, Sato E, Hattori T, Nakashima T. Detection of human cytomegalovirus DNA in perilymph of patients with sensorineural hearing loss using real‐time PCR. Journal of medical virology. 2003 Jan 1;69(1):72-5. https://doi.org/10.1002/jmv.10... PMid:12436480.
6.
Soleymani M, Nikakhlagh S, Hafezi G, Albokordi M, Saki N. Comparison of communication and social skills abilities of children with hearing impairment after cochlear implantation and parental expectations: A study conducted in Khuzestan cochlear implant center. International Journal of Pharmacy and Technology. 2016;8(3):14791-802.
7.
Ralli M, Rolesi R, Anzivino R, Turchetta R, Fetoni AR. Acquired sensory neural hearing loss in children current research therapeutic perspectives. Acta Otorhinolaryngol Ital. 2017;37(37):500-508. https://doi.org/10.14639/0392-... PMid:29327735 PMCid:PMC5782428.
8.
Bachor E, Sudhoff H, Litschel R, Karmody CS. The pathology of the temporal bones of a child with acquired cytomegalovirus infection: studies by light microscopy, immunohistochemistry and polymerase-chain reaction. International journal of pediatric otorhinolaryngology. 2000 Oct 16;55(3):215-24. https://doi.org/10.1016/S0165-....
9.
Tanaka N, Kimura H, Iida K, Saito Y, Tsuge I, Yoshimi A, Matsuyama T, Morishima T. Quantitative analysis of cytomegalovirus load using a real‐time PCR assay. Journal of medical virology. 2000 Apr 1;60(4):455-62. https://doi.org/10.1002/(SICI)...<455::AID-JMV14>3.0.CO;2-Q.
10.
Ciorba A, Bovo R, Trevisi P, Bianchini C, Arboretti R, Martini A. Rehabilitation and outcome of severe profound deafness in a group of 16 infants affected by congenital cytomegalovirus infection. European Archives of Oto-Rhino-Laryngology. 2009;266(10):1539-1546. https://doi.org/10.1007/s00405... PMid:19283400.
11.
Mizuno T, Sugiura S, Kimura H, Ando Y, Sone M, Nishiyama Y, Nakashima T. Detection of cytomegalovirus DNA in preserved umbilical cords from patients with sensorineural hearing loss. European Archives of Oto-Rhino-Laryngology. 2009;266(3):351–5. https://doi.org/10.1007/s00405... PMid:18563424.
12.
Nikakhlagh S, Yadollahpour A, Karimi M, Bagheripour H, Hematipour S, Malehi AS, Saki N. Investigating Gender Differences on the Age of Suspicion of Children with Hearing Loss in Iran. International Journal of Mental Health and Addiction. 2017;15(2): 271-6. https://doi.org/10.1007/s11469....
13.
Di Nardo W, Cattani P, Scorpecci A, Giannantonio S, D’Onghia S, Fadda G, Paludetti G. Cytomegalovirus DNA retrieval in the inner ear fluids of a congenitally deaf child one month after primary infection: a case report. The Laryngoscope. 2011 Apr 1;121 (4):828-30. https://doi.org/10.1002/lary.2... PMid:21305557.
14.
Noyola DE, Demmler GJ, Williamson WD, Griesser C, Sellers S, Llorente A, Littman T, Williams S, Jarrett L, Yow MD. Congenital CMV Longitudinal Study Group. Cytomegalovirus urinary excretion and long term outcome in children with congenital cytomegalovirus infection. The Pediatric infectious disease journal. 2000 Jun 1;19(6):505-10. https://doi.org/10.1097/000064... PMid:10877163.
15.
Davis GL. Cytomegalovirus in the inner ear. Case report and electron microscopic study. The Annals of otology, rhinology, and laryngology. 1969 Dec;78(6):1179-88. https://doi.org/10.1177/000348... PMid:4312920.
16.
Bauer PW, Parizi‐Robinson M, Roland PS, Yegappan S. Cytomegalovirus in the perilymphatic fluid. The Laryngoscope. 2005 Feb 1;115(2):223-5. https://doi.org/10.1097/01.mlg... PMid:15689739.
17.
Noorbakhsh S, Farhadi M, Daneshi A, Mohamadi S, Tabatabaei A. Viral infections detected by serology and PCR of perilymphatic fluid in children with idiopathic sensorineural hearing loss. Eastern Mediterranean Health Journal;2011: Vol. 17 No. 11:868-71. https://doi.org/10.26719/2011.....
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.